2pmi Summary

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Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway with Bound ATP-gamma-S

The structure was published by Huang, K., Ferrin-O'Connell, I., Zhang, W., Leonard, G.A., O'Shea, E.K., and Quiocho, F.A., in 2007 in a paper entitled "Structure of the Pho85-Pho80 CDK-Cyclin Complex of the Phosphate-Responsive Signal Transduction Pathway" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.9 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cyclin-dependent protein kinase PHO85 and PHO85 cyclin PHO80.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cyclin-dependent protein kinase PHO85 P17157 (1-305) (PHO85_YEAST)search Saccharomyces cerevisiae S288csearch 100% 317 89%
C Cyclin-dependent protein kinase PHO85 P17157 (1-305) (PHO85_YEAST)search Saccharomyces cerevisiae S288csearch 100% 317 89%
B PHO85 cyclin PHO80 P20052 (1-293) (PHO80_YEAST)search Saccharomyces cerevisiae S288csearch 100% 293 73%
D PHO85 cyclin PHO80 P20052 (1-293) (PHO80_YEAST)search Saccharomyces cerevisiae S288csearch 100% 293 73%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P17157 (1 - 305) Cyclin-dependent protein kinase PHO85 Saccharomyces cerevisiae
P20052 (1 - 293) PHO85 cyclin PHO80 Saccharomyces cerevisiae

Chain Structural classification (CATH) Sequence family (Pfam)
A, C (P17157) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch
B, D (P20052) PF08613: Cyclinsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, C (P17157) protein kinase activitysearch protein bindingsearch cyclin-dependent protein serine/threonine kinase activitysearch nucleotide bindingsearch protein serine/threonine kinase activitysearch ATP bindingsearch transferase activity, transferring phosphorus-containing groupssearch kinase activitysearch transferase activitysearch regulation of cell cyclesearch protein phosphorylationsearch negative regulation of transcription from RNA polymerase II promotersearch regulation of transcription involved in G1/S transition of mitotic cell cyclesearch regulation of protein stabilitysearch cellular response to DNA damage stimulussearch positive regulation of macroautophagysearch negative regulation of macroautophagysearch negative regulation of phosphate metabolic processsearch negative regulation of sequence-specific DNA binding transcription factor activitysearch regulation of establishment or maintenance of cell polaritysearch regulation of protein localizationsearch negative regulation of calcium-mediated signalingsearch negative regulation of glycogen biosynthetic processsearch phosphorylationsearch cyclin-dependent protein kinase holoenzyme complexsearch cytoplasmsearch nucleussearch
B, D (P20052) protein kinase bindingsearch cyclin-dependent protein serine/threonine kinase regulator activitysearch protein bindingsearch regulation of cyclin-dependent protein serine/threonine kinase activitysearch regulation of protein localizationsearch negative regulation of calcium-mediated signalingsearch negative regulation of transcription from RNA polymerase II promotersearch negative regulation of macroautophagysearch cellular metal ion homeostasissearch negative regulation of phosphate metabolic processsearch cytoplasmsearch nucleussearch cyclin-dependent protein kinase holoenzyme complexsearch

Chain InterPro annotation
A, C Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
B, D Cyclin PHO80-likesearch