2pm9

X-ray diffraction
3.3Å resolution

Crystal structure of yeast Sec13/31 vertex element of the COPII vesicular coat

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and organization of coat proteins in the COPII cage.
Cell 129 1325-36 (2007)
PMID: 17604721

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-153783 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein transport protein SEC31 Chain: A
Molecule details ›
Chain: A
Length: 416 amino acids
Theoretical weight: 45.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Hi5 insect cells
UniProt:
  • Canonical: P38968 (Residues: 1-411; Coverage: 32%)
Gene names: D1229, SEC31, WEB1, YDL195W
Sequence domains: WD domain, G-beta repeat
Structure domains:
Protein transport protein SEC13 Chain: B
Molecule details ›
Chain: B
Length: 297 amino acids
Theoretical weight: 33.19 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Hi5 insect cells
UniProt:
  • Canonical: Q04491 (Residues: 1-297; Coverage: 100%)
Gene names: ANU3, L8167.4, SEC13, YLR208W
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43
Unit cell:
a: 155.163Å b: 155.163Å c: 59.866Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.251 0.305
Expression system: Hi5 insect cells