› 2plm

X-ray diffraction
2.1Å resolution

Crystal structure of the protein TM0936 from Thermotoga maritima complexed with ZN and S-inosylhomocysteine

Released:
DOI: 10.2210/pdb2plm/pdb
PDB entry 2plm coloured by chain, front view.
PDB entry 2plm coloured by chain, side view.
PDB entry 2plm coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structure-based activity prediction for an enzyme of unknown function.
Hermann JC, Marti-Arbona R, Fedorov AA, Fedorov E, Almo SC, Shoichet BK, Raushel FM
Nature 448 775-9 (2007)
PMID: 17603473

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-homocysteine + H(2)O = S-inosyl-L-homocysteine + NH(3)
S-methyl-5'-thioadenosine + H(2)O = S-methyl-5'-thioinosine + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-194672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-methylthioadenosine/S-adenosylhomocysteine deaminase Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 45.82 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X034 (Residues: 1-406; Coverage: 100%)
Gene names: TM_0936, mtaD
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand SIB 1 x SIB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P3221
Unit cell:
a: 113.278Å b: 113.278Å c: 80.298Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.209 0.238
Expression system: Escherichia coli

Quick links

Citations

22 review citations

'Unknown' proteins and 'orphan' enzymes: the missing half of the engineering parts list--and how to find it.
Hanson et al. (2009)
21 more

11 mentions without citation

Leveraging structure for enzyme function prediction: methods, opportunities, and challenges.
Jacobson et al. (2014)
10 more