2pi5 Summary


T7 RNA polymerase complexed with a phi10 promoter

The structure was published by Kennedy, W.P., Momand, J.R., and Yin, Y.W., in 2007 in a paper entitled "Mechanism for de novo RNA synthesis and initiating nucleotide specificity by t7 RNA polymerase." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.9 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely 5'-D(*CP*TP*TP*C*CP*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP*AP*TP*TP*A)-3', 5'-D(*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*T)-3', and DNA-directed RNA polymerase.

The molecule most likely forms heterotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A DNA-directed RNA polymerase P00573 (6-883) (RPOL_BPT7)search Enterobacteria phage T7search 99% 878 98%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P00573 (6 - 883) DNA-directed RNA polymerase Enterobacteria phage T7

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (P00573) T7 RNA polymerasesearch T7 RNA polymerase, domain 1search, Helix Hairpinssearch, Alpha-Beta Plaitssearch, 5' to 3' exonuclease, C-terminal subdomainsearch PF00940: DNA-dependent RNA polymerasesearch, PF14700: DNA-directed RNA polymerase N-terminalsearch

Chain ID Molecular function (GO) Biological process (GO)
A (P00573) DNA-directed RNA polymerase activitysearch DNA bindingsearch transferase activitysearch nucleotidyltransferase activitysearch transcription, DNA-templatedsearch

Chain InterPro annotation
A DNA-directed RNA polymerase, phage-typesearch DNA-directed RNA polymerase, helix hairpin domainsearch DNA-directed RNA polymerase N-terminal domainsearch