› 2phh

X-ray diffraction
2.7Å resolution

THE COENZYME ANALOGUE ADENOSINE 5-DIPHOSPHORIBOSE DISPLACES FAD IN THE ACTIVE SITE OF P-HYDROXYBENZOATE HYDROXYLASE. AN X-RAY CRYSTALLOGRAPHIC INVESTIGATION

Released:
DOI: 10.2210/pdb2phh/pdb
PDB entry 2phh coloured by chain, front view.
PDB entry 2phh coloured by chain, side view.
PDB entry 2phh coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
The coenzyme analogue adenosine 5-diphosphoribose displaces FAD in the active site of p-hydroxybenzoate hydroxylase. An x-ray crystallographic investigation.
van der Laan JM, Schreuder HA, Swarte MB, Wierenga RK, Kalk KH, Hol WG, Drenth J
Biochemistry 28 7199-205 (1989)
PMID: 2819062

Function and Biology Details

Reaction catalysed: 
4-hydroxybenzoate + NADPH + O(2) = protocatechuate + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
p-hydroxybenzoate hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 44.38 KDa
Source organism: Pseudomonas fluorescens
Expression system: Not provided
UniProt:
  • Canonical: P00438 (Residues: 1-394; Coverage: 100%)
Gene name: pobA
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand APR 1 x APR
Ligand PHB 1 x PHB
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 71.7Å b: 146.4Å c: 89.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 not available not available
Expression system: Not provided

Quick links

Citations

1 review citation

Mechanistic aspects of carotenoid biosynthesis.
Moise et al. (2014)
10 other articles

8 mentions without citation

LIGSITEcsc: predicting ligand binding sites using the Connolly surface and degree of conservation.
Huang et al. (2006)
7 more