› 2pcn

X-ray diffraction
1.9Å resolution

Crystal structure of S-adenosylmethionine: 2-dimethylmenaquinone methyltransferase (gk_1813) from geobacillus kaustophilus HTA426

Released:
DOI: 10.2210/pdb2pcn/pdb
PDB entry 2pcn coloured by chain, front view.
PDB entry 2pcn coloured by chain, side view.
PDB entry 2pcn coloured by chain, top view.
Source organism: Geobacillus kaustophilus HTA426
Entry authors: Jeyakanthan J, Kanaujia SP, Rafi ZA, Sekar K, Agari Y, Ebihara A, Kuramitsu S, Shinkai A, Shiro Y, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reactions catalysed: 
Oxaloacetate = pyruvate + CO(2)
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate = oxaloacetate + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-4-methyl-2-oxoglutarate aldolase Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 17.08 KDa
Source organism: Geobacillus kaustophilus HTA426
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5KYY8 (Residues: 1-161; Coverage: 100%)
Gene name: GK1813
Sequence domains: Aldolase/RraA
Structure domains: Ribonuclease E inhibitor RraA/RraA-like

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45PX
Spacegroup: R3
Unit cell:
a: 84.573Å b: 84.573Å c: 83.338Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.176 0.205
Expression system: Escherichia coli

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