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CRYSTAL STRUCTURE OF A COMPLEX BETWEEN ELECTRON TRANSFER PARTNERS, CYTOCHROME C PEROXIDASE AND CYTOCHROME C

X-ray diffraction
2.3Å resolution
PDB entry 2pcc coloured by chain, front view.
PDB entry 2pcc coloured by chain, side view.
PDB entry 2pcc coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Cytochrome c isoform 1 and Cytochrome c peroxidase, mitochondrial
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Multimeric state: hetero dimer
Accessible surface area: 17211.21 Å2
Buried surface area: 3861.08 Å2
Dissociation area: 600.51 Å2
Dissociation energy (ΔGdiss): -7.04 kcal/mol
Dissociation entropy (TΔSdiss): 11.62 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-132231
Assembly 2
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Assembly Name: Cytochrome c isoform 1 and Cytochrome c peroxidase, mitochondrial
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Multimeric state: hetero dimer
Accessible surface area: 17267.19 Å2
Buried surface area: 3799.2 Å2
Dissociation area: 568.09 Å2
Dissociation energy (ΔGdiss): -6.53 kcal/mol
Dissociation entropy (TΔSdiss): 11.6 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-132231

Macromolecules

Cytochrome c peroxidase, mitochondrial
Chains: A, C
Length: 296 amino acids
Theoretical weight: 33.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P00431 (Residues: 68-361; Coverage: 81%)
Gene names: CCP, CCP1, CPO, YKR066C
Pfam: Peroxidase
InterPro:
CATH:
SCOP: CCP-like
Cytochrome c peroxidase, mitochondrial in PDB entry 2pcc, assembly 1, front view.
Cytochrome c peroxidase, mitochondrial in PDB entry 2pcc, assembly 1, side view.
Cytochrome c peroxidase, mitochondrial in PDB entry 2pcc, assembly 1, top view.
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Cytochrome c isoform 1
Chains: B, D
Length: 108 amino acids
Theoretical weight: 12.07 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P00044 (Residues: 2-109; Coverage: 99%)
Gene names: CYC1, J1653, YJR048W
Pfam: Cytochrome c
InterPro:
CATH: Cytochrome c-like domain
SCOP: monodomain cytochrome c
Cytochrome c isoform 1 in PDB entry 2pcc, assembly 1, front view.
Cytochrome c isoform 1 in PDB entry 2pcc, assembly 1, side view.
Cytochrome c isoform 1 in PDB entry 2pcc, assembly 1, top view.
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