› 2p87

X-ray diffraction
2.3Å resolution

Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8

Released:
DOI: 10.2210/pdb2p87/pdb
PDB entry 2p87 coloured by chain, front view.
PDB entry 2p87 coloured by chain, side view.
PDB entry 2p87 coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutants.
Zhang L, Shen J, Guarnieri MT, Heroux A, Yang K, Zhao R
Protein Sci 16 1024-31 (2007)
PMID: 17473007

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-splicing factor 8 homolog Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 31.2 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P34369 (Residues: 2057-2329; Coverage: 12%)
Gene names: C50C3.6, prp-8
Sequence domains:
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P41212
Unit cell:
a: 65.063Å b: 65.063Å c: 135.552Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.249
Expression system: Escherichia coli

Quick links

Citations

16 review citations

The spliceosome: design principles of a dynamic RNP machine.
Wahl et al. (2009)
15 more

1 mention without citation

Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutants.
Zhang et al. (2007)