› 2p7j

X-ray diffraction
2.25Å resolution

Crystal structure of the domain of putative sensory box/GGDEF family protein from Vibrio parahaemolyticus

Released:
DOI: 10.2210/pdb2p7j/pdb
PDB entry 2p7j coloured by chain, front view.
PDB entry 2p7j coloured by chain, side view.
PDB entry 2p7j coloured by chain, top view.
Source organism: Vibrio parahaemolyticus RIMD 2210633
Entry authors: Wu R, James A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative sensory box/GGDEF family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 33.31 KDa
Source organism: Vibrio parahaemolyticus RIMD 2210633
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q87SR8 (Residues: 27-312; Coverage: 47%)
Gene name: VP0354
Sequence domains: Bacterial histidine kinase, sensor domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand NA 1 x NA
Ligand ACY 1 x ACY
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 72.98Å b: 81.596Å c: 121.851Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.239
Expression system: Escherichia coli BL21

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