2p6b Summary

pdbe.org/2p6b
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Crystal Structure of Human Calcineurin in Complex with PVIVIT Peptide

The structure was published by Li, H., Zhang, L., Rao, A., Harrison, S.C., and Hogan, P.G., in 2007 in a paper entitled "Structure of calcineurin in complex with PVIVIT peptide: Portrait of a low-affinity signalling interaction" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.3 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely PVIVIT 14-mer Peptide, Calmodulin-dependent calcineurin A subunit alpha isoform, and Calcineurin subunit B isoform 1.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heteropentamers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
E PVIVIT 14-mer Peptide Not available
Synthetic Not available 15 93%
A Calmodulin-dependent calcineurin A subunit alpha isoform Q08209 (1-380) (PP2BA_HUMAN)search Homo sapienssearch 100% 383 93%
C Calmodulin-dependent calcineurin A subunit alpha isoform Q08209 (1-380) (PP2BA_HUMAN)search Homo sapienssearch 100% 383 93%
B Calcineurin subunit B isoform 1 P63098 (16-170) (CANB1_HUMAN)search Homo sapienssearch 91% 156 98%
D Calcineurin subunit B isoform 1 P63098 (16-170) (CANB1_HUMAN)search Homo sapienssearch 91% 156 98%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
Q08209 (1 - 380) Calmodulin-dependent calcineurin A subunit alpha isoform Homo sapiens
P63098 (16 - 170) Calcineurin subunit B isoform 1 Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
E
A, C (Q08209) Protein serine/threonine phosphatasesearch Purple Acid Phosphatase; chain A, domain 2search PF00149: Calcineurin-like phosphoesterasesearch
B, D (P63098) Calmodulin-likesearch PF13499: EF-hand domain pairsearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
A, C (Q08209) hydrolase activitysearch
B, D (P63098) calcium ion bindingsearch protein domain specific bindingsearch calmodulin bindingsearch protein bindingsearch calcium-dependent protein serine/threonine phosphatase activitysearch metal ion bindingsearch cytosolsearch plasma membranesearch sarcolemmasearch calcineurin complexsearch cytoplasmsearch membranesearch nucleoplasmsearch apoptotic processsearch intrinsic apoptotic signaling pathwaysearch innate immune responsesearch positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathwaysearch dephosphorylationsearch Fc-epsilon receptor signaling pathwaysearch

Chain InterPro annotation
E
A, C Calcineurin-like phosphoesterase domain, apaH typesearch Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatasesearch Metallo-dependent phosphatase-likesearch
B, D EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch