spacer
EBI PDBe PDBeView

PDBe Entry: 2oww view

Covalent intermediate in amylomaltase in complex with the acceptor analog 4-deoxyglucose
Summary
Header Transferasesearch
Method X-RAY DIFFRACTION
Experiment Resolution: 2.2 Å, R-factor: 20.019%, Free R-factor: 23.968%, Spacegroup: P 31 2 1
Released 03/04/2007, deposition: 17/02/2007, last revision: 24/02/2009
Authors Barends, T.R.M.search; Bultema, J.B.search; Kaper, T.search; van der Maarel, M.J.E.C.search; Dijkhuizen, L.search; Dijkstra, B.W.search
Primary citation Three-way stabilization of the covalent intermediate in amylomaltase, an alpha-amylase-like transglycosylase.
J.BIOL.CHEM.search vol:282, pag:17242-17249 (2007) [PubMed ID 17420245 ]search
Keywords alpha-amylasesearch, covalent intermediatesearch, beta-alpha barrelsearch, Transferasesearch
EC 2.4.1.25 ExPASy BRENDA search (A)
Organism Thermus thermophilus 274search(A)
UniProt 4-alpha-glucanotransferase (EC 2.4.1.25) (Amylomaltase) (Disproportionating enzyme) (D-enzyme) O87172search (A)
Solvent A
Related entries 1esw, 2owc
Polymers
Id Name Type UniProt Residues Observed
A 4-alpha-glucanotransferase Protein O87172 (MALQ_THETH)search
 502 99%
Heterogens
Id Name Ligands
A SUGAR (ACARBOSE) ACR search
A SUGAR (4-DEOXY-ALPHA-D-GLUCOSE) G4D search
A MALONATE ION MLI search
A GLYCEROL GOL search
spacer