2owc

X-ray diffraction
2.05Å resolution

Structure of a covalent intermediate in Thermus thermophilus amylomaltase

Released:

Function and Biology Details

Reaction catalysed:
Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-131491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
4-alpha-glucanotransferase Chain: A
Molecule details ›
Chain: A
Length: 502 amino acids
Theoretical weight: 57.53 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O87172 (Residues: 1-500; Coverage: 100%)
Gene name: malQ
Sequence domains: 4-alpha-glucanotransferase
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, AC1
2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3121
Unit cell:
a: 92.405Å b: 92.405Å c: 154.115Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.216
Expression system: Escherichia coli BL21(DE3)