› 2ovp

X-ray diffraction
2.9Å resolution

Structure of the Skp1-Fbw7 complex

Released:
DOI: 10.2210/pdb2ovp/pdb
PDB entry 2ovp coloured by chain, front view.
PDB entry 2ovp coloured by chain, side view.
PDB entry 2ovp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a Fbw7-Skp1-cyclin E complex: multisite-phosphorylated substrate recognition by SCF ubiquitin ligases.
Hao B, Oehlmann S, Sowa ME, Harper JW, Pavletich NP
Mol Cell 26 131-43 (2007)
PMID: 17434132

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-159044 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
S-phase kinase-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63208 (Residues: 1-69, 82-163; Coverage: 89%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A
F-box/WD repeat-containing protein 7 Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q969H0 (Residues: 263-707; Coverage: 63%)
Gene names: FBW7, FBX30, FBXW7, SEL10
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: I4122
Unit cell:
a: 222.702Å b: 222.702Å c: 102.883Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.268
Expression system: Escherichia coli BL21

Quick links

Citations

85 review citations

RING domain E3 ubiquitin ligases.
Deshaies et al. (2009)
84 more

17 mentions without citation

The molecular logic of Notch signaling--a structural and biochemical perspective.
Gordon et al. (2008)
16 more