› 2ori

X-ray diffraction
1.8Å resolution

Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (A193V/Q199R/)

Released:
DOI: 10.2210/pdb2ori/pdb
PDB entry 2ori coloured by chain, front view.
PDB entry 2ori coloured by chain, side view.
PDB entry 2ori coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Counago R, Shamoo Y, Wilson CJ, Wu G, Myers J

Function and Biology Details

Reaction catalysed: 
ATP + AMP = 2 ADP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147679 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 24.08 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16304 (Residues: 1-216; Coverage: 100%)
Gene names: BSU01370, adk
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 2 x MG
Ligand AP5 2 x AP5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 34.694Å b: 75.136Å c: 77.523Å
α: 90° β: 98.37° γ: 90°
R-values:
R R work R free
0.228 0.228 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links