› 2orc

Solution NMR

CRO REPRESSOR INSERTION MUTANT K56-[DGEVK], NMR, 32 STRUCTURES

Released:
DOI: 10.2210/pdb2orc/pdb
PDB entry 2orc coloured by chain, ensemble of 32 models, front view.
PDB entry 2orc coloured by chain, ensemble of 32 models, side view.
PDB entry 2orc coloured by chain, ensemble of 32 models, top view.
Source organism: Lambdavirus lambda
Primary publication:
Solution structure and dynamics of a designed monomeric variant of the lambda Cro repressor.
Mossing MC
Protein Sci 7 983-93 (1998)
PMID: 9568905

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein cro Chain: A
Molecule details ›
Chain: A
Length: 71 amino acids
Theoretical weight: 7.91 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03040 (Residues: 1-53, 54-66; Coverage: 100%)
Gene names: cro, lambdap57
Sequence domains: Cro
Structure domains: CRO Repressor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DISTANCE GEOMETRY/ SIMULATED ANNEALING
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Crystal structure of an engineered Cro monomer bound nonspecifically to DNA: possible implications for nonspecific binding by the wild-type protein.
Albright et al. (1998)
6 more

2 mentions without citation

Systematic comparison of crystal and NMR protein structures deposited in the protein data bank.
Sikic et al. (2010)
1 more