› 2or3

X-ray diffraction
1.2Å resolution

Pre-oxidation Complex of Human DJ-1

Released:
DOI: 10.2210/pdb2or3/pdb
PDB entry 2or3 coloured by chain, front view.
PDB entry 2or3 coloured by chain, side view.
PDB entry 2or3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cysteine pKa depression by a protonated glutamic acid in human DJ-1.
Witt AC, Lakshminarasimhan M, Remington BC, Hasim S, Pozharski E, Wilson MA
Biochemistry 47 7430-40 (2008)
PMID: 18570440

Function and Biology Details

Reaction catalysed: 
An N(6)-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H(2)O = a [protein]-L-lysine + lactate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Parkinson disease protein 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 19.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99497 (Residues: 1-189; Coverage: 100%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 43.914Å b: 85.766Å c: 98.829Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.136 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Cysteine-mediated redox signaling: chemistry, biology, and tools for discovery.
Paulsen et al. (2013)
15 more

9 mentions without citation

Cysteine pKa depression by a protonated glutamic acid in human DJ-1.
Witt et al. (2008)
8 more