› 2oly

X-ray diffraction
1.7Å resolution

Structure of human insulin in presence of urea at pH 7.0

Released:
DOI: 10.2210/pdb2oly/pdb
PDB entry 2oly coloured by chain, front view.
PDB entry 2oly coloured by chain, side view.
PDB entry 2oly coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic characterization of two novel crystal forms of human insulin induced by chaotropic agents and a shift in pH.
Norrman M, Schluckebier G
BMC Struct Biol 7 83 (2007)
PMID: 18093308

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134235 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

5 bound ligands:
Ligand RCO 8 x RCO
Ligand URE 7 x URE
Ligand ZN 2 x ZN
Ligand CL 5 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: C2
Unit cell:
a: 100.24Å b: 60.15Å c: 62.92Å
α: 90° β: 116.21° γ: 90°
R-values:
R R work R free
0.186 0.184 0.221

Quick links

Citations

4 review citations

Inside the Insulin Secretory Granule.
Germanos et al. (2021)
3 more

2 mentions without citation

Predicting binding sites from unbound versus bound protein structures.
Clark et al. (2020)
1 more