› 2olv

X-ray diffraction
2.8Å resolution

Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex

Released:
DOI: 10.2210/pdb2olv/pdb
PDB entry 2olv coloured by chain, front view.
PDB entry 2olv coloured by chain, side view.
PDB entry 2olv coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural insight into the transglycosylation step of bacterial cell-wall biosynthesis.
Lovering AL, de Castro LH, Lim D, Strynadka NC
Science 315 1402-5 (2007)
PMID: 17347437

Function and Biology Details

Reaction catalysed: 
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193115 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
peptidoglycan glycosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 669 amino acids
Theoretical weight: 74.64 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9R744 (Residues: 60-727; Coverage: 92%)
Gene names: EP54_02360, EQ90_10185, GO814_11815, GO942_13395, H2639_02150, HMPREF3211_02644, M1K003_0294, NCTC10702_02314, SAMEA70153168_00413, pbp2, ponA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand M0E 2 x M0E
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21212
Unit cell:
a: 79.565Å b: 212.211Å c: 91.635Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.231 0.28
Expression system: Escherichia coli

Quick links

Citations

51 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
50 more

12 mentions without citation

Targeted isolation and cultivation of uncultivated bacteria by reverse genomics.
Cross et al. (2019)
11 more