› 2ol1

X-ray diffraction
1.8Å resolution

High Resolution Crystal Structures of Vaccinia Virus dUTPase

Released:
DOI: 10.2210/pdb2ol1/pdb
PDB entry 2ol1 coloured by chain, front view.
PDB entry 2ol1 coloured by chain, side view.
PDB entry 2ol1 coloured by chain, top view.
Source organism: Vaccinia virus
Primary publication:
Structures of vaccinia virus dUTPase and its nucleotide complexes.
Samal A, Schormann N, Cook WJ, DeLucas LJ, Chattopadhyay D
Acta Crystallogr D Biol Crystallogr 63 571-80 (2007)
PMID: 17452782

Function and Biology Details

Reaction catalysed: 
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyuridine 5'-triphosphate nucleotidohydrolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 147 amino acids
Theoretical weight: 16.32 KDa
Source organism: Vaccinia virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P17374 (Residues: 1-147; Coverage: 100%)
Gene names: DUT, F2L, OPG046, VACWR041
Sequence domains: dUTPase
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand UMP 3 x UMP
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P65
Unit cell:
a: 119.97Å b: 119.97Å c: 50.13Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.231
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Targeting Nucleotide Biosynthesis: A Strategy for Improving the Oncolytic Potential of DNA Viruses.
Irwin et al. (2017)
1 more

1 mention without citation

Poxvirus proteomics and virus-host protein interactions.
Van Vliet et al. (2009)