› 2okk

X-ray diffraction
2.3Å resolution

The X-ray crystal structure of the 65kDa isoform of Glutamic Acid Decarboxylase (GAD65)

Released:
DOI: 10.2210/pdb2okk/pdb
PDB entry 2okk coloured by chain, front view.
PDB entry 2okk coloured by chain, side view.
PDB entry 2okk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop.
Fenalti G, Law RH, Buckle AM, Langendorf C, Tuck K, Rosado CJ, Faux NG, Mahmood K, Hampe CS, Banga JP, Wilce M, Schmidberger J, Rossjohn J, El-Kabbani O, Pike RN, Smith AI, Mackay IR, Rowley MJ, Whisstock JC
Nat Struct Mol Biol 14 280-6 (2007)
PMID: 17384644

Function and Biology Details

Reaction catalysed: 
L-glutamate = 4-aminobutanoate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate decarboxylase 2 Chain: A
Molecule details ›
Chain: A
Length: 497 amino acids
Theoretical weight: 56.78 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q05329 (Residues: 88-584; Coverage: 85%)
Gene names: GAD2, GAD65
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ABU 2 x ABU
Ligand GOL 1 x GOL
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2221
Unit cell:
a: 78.251Å b: 99.057Å c: 120.009Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.196 0.256
Expression system: Saccharomyces cerevisiae

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Citations

29 review citations

Development of GABA innervation in the cerebral and cerebellar cortices.
Huang et al. (2007)
28 more

12 mentions without citation

Exploring the role of Mycobacterium avium subspecies paratuberculosis in the pathogenesis of type 1 diabetes mellitus: a pilot study.
Naser et al. (2013)
11 more