› 2ojp

X-ray diffraction
1.7Å resolution

The crystal structure of a dimeric mutant of Dihydrodipicolinate synthase from E.coli- DHDPS-L197Y

Released:
DOI: 10.2210/pdb2ojp/pdb
PDB entry 2ojp coloured by chain, front view.
PDB entry 2ojp coloured by chain, side view.
PDB entry 2ojp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Evolution of quaternary structure in a homotetrameric enzyme.
Griffin MD, Dobson RC, Pearce FG, Antonio L, Whitten AE, Liew CK, Mackay JP, Trewhella J, Jameson GB, Perugini MA, Gerrard JA
J Mol Biol 380 691-703 (2008)
PMID: 18556019

Function and Biology Details

Reaction catalysed: 
Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141376 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-tetrahydrodipicolinate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 31.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6L2 (Residues: 1-292; Coverage: 100%)
Gene names: JW2463, b2478, dapA
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
2 modified residues:
Ligand CSD 2 x CSD
Ligand KGC 2 x KGC

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 60.482Å b: 106.705Å c: 120.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.228
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The role of quaternary structure in (beta/alpha)(8)-barrel proteins: evolutionary happenstance or a higher level of structure-function relationships?
Devenish et al. (2009)
1 more

3 mentions without citation

Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.
Naz et al. (2023)
2 more