› 2ofr

X-ray diffraction
1Å resolution

1.00 A Crystal Structure Of V36A/D129A/L130A Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed With Nitric Oxide at pH 5.6

Released:
DOI: 10.2210/pdb2ofr/pdb
PDB entry 2ofr coloured by chain, front view.
PDB entry 2ofr coloured by chain, side view.
PDB entry 2ofr coloured by chain, top view.
Source organism: Rhodnius prolixus
Entry author: Amoia AM

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: X
Molecule details ›
Chain: X
Length: 184 amino acids
Theoretical weight: 20.18 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand PO4 1 x PO4
Ligand NO 1 x NO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: C2
Unit cell:
a: 70.191Å b: 42.637Å c: 52.975Å
α: 90° β: 94.12° γ: 90°
R-values:
R R work R free
0.127 0.126 0.148
Expression system: Escherichia coli BL21(DE3)

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