› 2ofk

X-ray diffraction
1.5Å resolution

Crystal Structure of 3-methyladenine DNA glycosylase I (TAG)

Released:
DOI: 10.2210/pdb2ofk/pdb
PDB entry 2ofk coloured by chain, front view.
PDB entry 2ofk coloured by chain, side view.
PDB entry 2ofk coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhi
Primary publication:
DNA damage recognition and repair by 3-methyladenine DNA glycosylase I (TAG).
Metz AH, Hollis T, Eichman BF
EMBO J 26 2411-20 (2007)
PMID: 17410210

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alkylated DNA, releasing 3-methyladenine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-3-methyladenine glycosylase I Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.82 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8Z2A5 (Residues: 1-183; Coverage: 95%)
Gene names: CAJ76_00885, CAJ80_00405, CAL67_00245, CB224_00605, CC884_01545, CC907_00250, CC972_01275, D4Z37_03335, D6Q30_05880, DKA88_00265, DNM71_00915, DNV09_00265, DS261_00915, DUQ91_00915, DUV11_00915, DVG36_00265, EBC38_00940, EID90_00270, ELQ62_00260, EU445_00905, EVI00_03490, F9R11_03430, F9Y21_03390, FI137_10725, G2227_03345, G2244_00905, G3982_000052, G3V49_000049, G4I71_000098, G4L28_001120, G4P30_000179, G4Y41_000076, G4Y55_000664, G9264_000049, G9C68_000537, G9X37_000032, GDI29_03430, GND71_000179, K3U68_18870, STY4160, ZZ17_00290, t3877, tag
Sequence domains: Methyladenine glycosylase
Structure domains: Hypothetical protein; domain 2

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand PGE 8 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 57.455Å b: 63.681Å c: 62.127Å
α: 90° β: 106.94° γ: 90°
R-values:
R R work R free
0.163 0.161 0.196
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Base excision repair.
Krokan et al. (2013)
5 more

3 mentions without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)
2 more