Function and Biology Details
Reaction catalysed:
(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero hexamer (preferred)
Assembly name:
PCNA homotrimer and DNA ligase 1 (preferred)
PDBe Complex ID:
PDB-CPX-138198 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.94 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 258 amino acids
Theoretical weight: 28.94 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P15873 (Residues: 1-258; Coverage: 100%)
Sequence domains:
- Proliferating cell nuclear antigen, N-terminal domain
- Proliferating cell nuclear antigen, C-terminal domain
DNA ligase 1
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.46 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
Length: 22 amino acids
Theoretical weight: 2.46 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
- Canonical: P04819 (Residues: 32-53; Coverage: 3%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SSRL BEAMLINE BL9-1
Spacegroup:
I23
Expression systems:
- Escherichia coli BL21(DE3)
- Not provided
