2ocf Summary


Human estrogen receptor alpha ligand-binding domain in complex with estradiol and the E2#23 FN3 monobody

The structure was published by Koide, A., Abbatiello, S., Rothgery, L., and Koide, S., in 2002 in a paper entitled "Probing protein conformational changes in living cells by using designer binding proteins: application to the estrogen receptor." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.95 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Estrogen receptor and Fibronectin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotetramers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Estrogen receptor P03372 (298-595) (ESR1_HUMAN)search Homo sapienssearch < 90% 298 78%
D Fibronectin P02751 (1447-1540) (FINC_HUMAN)search Homo sapienssearch < 90% 121 76%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P03372 (298 - 595) Estrogen receptor Homo sapiens
P02751 (1447 - 1540) Fibronectin Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A Retinoid X Receptorsearch Ligand-binding domain of nuclear hormone receptorsearch, Oestrogen-type nuclear receptor final C-terminalsearch
D Immunoglobulinssearch Fibronectin type III domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (P03372) DNA bindingsearch sequence-specific DNA binding transcription factor activitysearch steroid hormone receptor activitysearch regulation of transcription, DNA-templatedsearch steroid hormone mediated signaling pathwaysearch nucleussearch

Chain InterPro annotation
A Nuclear hormone receptor, ligand-binding, coresearch Steroid hormone receptorsearch Nuclear hormone receptor, ligand-bindingsearch Oestrogen-type nuclear receptor final C-terminal domainsearch
D Fibronectin type IIIsearch Immunoglobulin-like foldsearch