› 2ob2

X-ray diffraction
1.92Å resolution

ppm1 in the absence of 1,8-ANS (cf 1JD)

Released:
DOI: 10.2210/pdb2ob2/pdb
PDB entry 2ob2 coloured by chain, front view.
PDB entry 2ob2 coloured by chain, side view.
PDB entry 2ob2 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A method for the general identification of protein crystals in crystallization experiments using a noncovalent fluorescent dye.
Groves MR, Müller IB, Kreplin X, Müller-Dieckmann J
Acta Crystallogr D Biol Crystallogr 63 526-35 (2007)
PMID: 17372358

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine = S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine methyl ester
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169993 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine carboxyl methyltransferase 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 327 amino acids
Theoretical weight: 37.62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q04081 (Residues: 2-328; Coverage: 100%)
Gene names: PPM1, YDR435C
Sequence domains: Leucine carboxyl methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 3 x SAH
2 bound ligands:
Ligand PO4 1 x PO4
Ligand GOL 2 x GOL
1 modified residue:
Ligand SMC 3 x SMC

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P65
Unit cell:
a: 110.62Å b: 110.62Å c: 161.94Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.206 0.268
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Extrinsic fluorescent dyes as tools for protein characterization.
Hawe et al. (2008)
1 more