› 2oap

X-ray diffraction
2.95Å resolution

Crystal structure of the archaeal secretion ATPase GspE in complex with AMP-PNP

Released:
DOI: 10.2210/pdb2oap/pdb
PDB entry 2oap coloured by chain, front view.
PDB entry 2oap coloured by chain, side view.
PDB entry 2oap coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Hexameric structures of the archaeal secretion ATPase GspE and implications for a universal secretion mechanism.
Yamagata A, Tainer JA
EMBO J 26 878-90 (2007)
PMID: 17255937

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial type II secretion system protein E domain-containing protein Chains: 1, 2
Molecule details ›
Chains: 1, 2
Length: 511 amino acids
Theoretical weight: 59.9 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O29598 (Residues: 1-511; Coverage: 100%)
Gene name: AF_0659
Sequence domains: Type II/IV secretion system protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: R32
Unit cell:
a: 132.864Å b: 132.864Å c: 442.261Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.226 0.234
Expression system: Escherichia coli

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Citations

14 review citations

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Putnam et al. (2007)
13 more

6 mentions without citation

Hexameric structures of the archaeal secretion ATPase GspE and implications for a universal secretion mechanism.
Yamagata et al. (2007)
5 more