› 2o8w

X-ray diffraction
1.86Å resolution

Crystal Structure and Binding Epitopes of Urokinase-type Plasminogen Activator (C122A/N145Q/S195A) in complex with Inhibitors

Released:
DOI: 10.2210/pdb2o8w/pdb
PDB entry 2o8w coloured by chain, front view.
PDB entry 2o8w coloured by chain, side view.
PDB entry 2o8w coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Zhao G, Yuan C, Jiang L, Huang Z, Huang M

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urokinase-type plasminogen activator chain B Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.43 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00749 (Residues: 179-431; Coverage: 62%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand PG4 1 x PG4
Ligand PL0 1 x PL0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: R3
Unit cell:
a: 120.875Å b: 120.875Å c: 42.591Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.213 0.255
Expression system: Komagataella pastoris

Quick links