2o25 Summary

pdbe.org/2o25
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Ubiquitin-Conjugating Enzyme E2-25 kDa Complexed With SUMO-1-Conjugating Enzyme UBC9

A publication describing this structure is not available. The depositing authors are Walker, J.R.search; Avvakumov, G.V.search; Xue, S.search; Newman, E.M.search; Mackenzie, F.search; Weigelt, J.search; Sundstrom, M.search; Arrowsmith, C.H.search; Edwards, A.M.search; Bochkarev, A.search; Dhe-Paganon, S.search; Structural Genomics Consortium (SGC)search

This crystal structure was determined using X-ray diffraction at a resolution of 2.6 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Ubiquitin-conjugating enzyme E2-25 kDa and SUMO-1-conjugating enzyme UBC9.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Ubiquitin-conjugating enzyme E2-25 kDa P61086 (1-200) (UBE2K_HUMAN)search Homo sapienssearch 94% 202 98%
B Ubiquitin-conjugating enzyme E2-25 kDa P61086 (1-200) (UBE2K_HUMAN)search Homo sapienssearch 94% 202 98%
C SUMO-1-conjugating enzyme UBC9 P63279 (1-158) (UBC9_HUMAN)search Homo sapienssearch 100% 160 98%
D SUMO-1-conjugating enzyme UBC9 P63279 (1-158) (UBC9_HUMAN)search Homo sapienssearch 100% 160 98%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P61086 (1 - 200) Ubiquitin-conjugating enzyme E2-25 kDa Homo sapiens
P63279 (1 - 158) SUMO-1-conjugating enzyme UBC9 Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B (P61086) UBA domainsearch, UBC-relatedsearch Ubiquitin Conjugating Enzymesearch PF00179: Ubiquitin-conjugating enzymesearch, PF00627: UBA/TS-N domainsearch
C, D (P63279) UBC-relatedsearch Ubiquitin Conjugating Enzymesearch PF00179: Ubiquitin-conjugating enzymesearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B (P61086) protein bindingsearch ubiquitin-ubiquitin ligase activitysearch nucleotide bindingsearch ubiquitin-protein transferase activitysearch ubiquitin protein ligase bindingsearch ligase activitysearch acid-amino acid ligase activitysearch ATP bindingsearch protein ubiquitinationsearch protein K48-linked ubiquitinationsearch ubiquitin-dependent protein catabolic processsearch intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stresssearch regulation of proteasomal ubiquitin-dependent protein catabolic processsearch extracellular vesicular exosomesearch cytoplasmsearch
C, D (P63279) acid-amino acid ligase activitysearch SUMO ligase activitysearch protein bindingsearch ubiquitin-protein transferase activitysearch RING-like zinc finger domain bindingsearch bHLH transcription factor bindingsearch poly(A) RNA bindingsearch protein C-terminus bindingsearch ATP bindingsearch enzyme bindingsearch transcription factor bindingsearch nucleotide bindingsearch ligase activitysearch HLH domain bindingsearch negative regulation of transcription from RNA polymerase II promotersearch post-translational protein modificationsearch protein sumoylationsearch cell cyclesearch negative regulation of transcription, DNA-templatedsearch mitotic nuclear divisionsearch protein ubiquitinationsearch positive regulation of sequence-specific DNA binding transcription factor activitysearch positive regulation of intracellular steroid hormone receptor signaling pathwaysearch viral processsearch chromosome segregationsearch regulation of receptor activitysearch ubiquitin-dependent protein catabolic processsearch cellular protein modification processsearch proteasome-mediated ubiquitin-dependent protein catabolic processsearch cell divisionsearch cellular protein metabolic processsearch nucleussearch nucleoplasmsearch fibrillar centersearch cytoplasmsearch synapsesearch nuclear bodysearch synaptonemal complexsearch PML bodysearch dendritesearch

Chain InterPro annotation
A, B Ubiquitin-associated domain/translation elongation factor EF-Ts, N-terminalsearch Ubiquitin-conjugating enzyme E2search UBA-likesearch Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryotesearch Ubiquitin-conjugating enzyme/RWD-likesearch Ubiquitin-conjugating enzyme, active sitesearch
C, D Ubiquitin-conjugating enzyme E2search Ubiquitin-conjugating enzyme/RWD-likesearch Ubiquitin-conjugating enzyme, active sitesearch SUMO-conjugating enzyme Ubc9search