2o25 Summary

pdbe.org/2o25
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Ubiquitin-Conjugating Enzyme E2-25 kDa Complexed With SUMO-1-Conjugating Enzyme UBC9

A publication describing this structure is not available. The depositing authors are Walker, J.R.search; Avvakumov, G.V.search; Xue, S.search; Newman, E.M.search; Mackenzie, F.search; Weigelt, J.search; Sundstrom, M.search; Arrowsmith, C.H.search; Edwards, A.M.search; Bochkarev, A.search; Dhe-Paganon, S.search; Structural Genomics Consortium (SGC)search

This crystal structure was determined using X-ray diffraction at a resolution of 2.6 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Ubiquitin-conjugating enzyme E2-25 kDa and SUMO-1-conjugating enzyme UBC9.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Ubiquitin-conjugating enzyme E2-25 kDa P61086 (1-200) (UBE2K_HUMAN)search Homo sapienssearch 94% 202 98%
B Ubiquitin-conjugating enzyme E2-25 kDa P61086 (1-200) (UBE2K_HUMAN)search Homo sapienssearch 94% 202 98%
C SUMO-1-conjugating enzyme UBC9 P63279 (1-158) (UBC9_HUMAN)search Homo sapienssearch 100% 160 98%
D SUMO-1-conjugating enzyme UBC9 P63279 (1-158) (UBC9_HUMAN)search Homo sapienssearch 100% 160 98%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P61086 (1 - 200) Ubiquitin-conjugating enzyme E2-25 kDa Homo sapiens
P63279 (1 - 158) SUMO-1-conjugating enzyme UBC9 Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B (P61086) UBA domainsearch, UBC-relatedsearch Ubiquitin Conjugating Enzymesearch, DNA helicase RuvA subunit, C-terminal domainsearch PF00179: Ubiquitin-conjugating enzymesearch, PF00627: UBA/TS-N domainsearch
C, D (P63279) UBC-relatedsearch Ubiquitin Conjugating Enzymesearch PF00179: Ubiquitin-conjugating enzymesearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, B (P61086) proteasome-mediated ubiquitin-dependent protein catabolic processsearch protein ubiquitinationsearch protein K48-linked ubiquitinationsearch ubiquitin-dependent protein catabolic processsearch intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stresssearch regulation of proteasomal ubiquitin-dependent protein catabolic processsearch protein bindingsearch nucleotide bindingsearch ubiquitin-like protein transferase activitysearch ubiquitin-protein transferase activitysearch ubiquitin protein ligase bindingsearch ATP bindingsearch ligase activitysearch acid-amino acid ligase activitysearch ubiquitin-ubiquitin ligase activitysearch ubiquitin protein ligase activitysearch cytoplasmsearch nucleussearch actin cytoskeletonsearch extracellular vesicular exosomesearch intermediate filament cytoskeletonsearch
C, D (P63279) viral processsearch ubiquitin-dependent protein catabolic processsearch cellular protein modification processsearch protein sumoylationsearch protein ubiquitinationsearch chromosome segregationsearch cellular protein metabolic processsearch negative regulation of transcription from RNA polymerase II promotersearch positive regulation of SUMO transferase activitysearch proteasome-mediated ubiquitin-dependent protein catabolic processsearch positive regulation of sequence-specific DNA binding transcription factor activitysearch post-translational protein modificationsearch cell divisionsearch positive regulation of intracellular steroid hormone receptor signaling pathwaysearch negative regulation of transcription, DNA-templatedsearch regulation of receptor activitysearch cell cyclesearch mitotic nuclear divisionsearch protein bindingsearch ubiquitin protein ligase bindingsearch ATP bindingsearch HLH domain bindingsearch SUMO conjugating enzyme activitysearch nucleotide bindingsearch SUMO transferase activitysearch acid-amino acid ligase activitysearch RING-like zinc finger domain bindingsearch ubiquitin protein ligase activitysearch protein C-terminus bindingsearch poly(A) RNA bindingsearch ligase activitysearch transcription factor bindingsearch bHLH transcription factor bindingsearch enzyme bindingsearch ubiquitin-protein transferase activitysearch nucleussearch cytoplasmsearch transferase complexsearch fibrillar centersearch PML bodysearch nuclear bodysearch dendritesearch synapsesearch nucleoplasmsearch sumoylated E2 ligase complexsearch synaptonemal complexsearch

Chain InterPro annotation
A, B Ubiquitin-associated domain/translation elongation factor EF-Ts, N-terminalsearch Ubiquitin-conjugating enzyme E2search UBA-likesearch Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryotesearch Ubiquitin-conjugating enzyme/RWD-likesearch Ubiquitin-conjugating enzyme, active sitesearch
C, D Ubiquitin-conjugating enzyme E2search Ubiquitin-conjugating enzyme/RWD-likesearch Ubiquitin-conjugating enzyme, active sitesearch SUMO-conjugating enzyme Ubc9search