› 2o20

X-ray diffraction
1.9Å resolution

Crystal structure of transcription regulator CcpA of Lactococcus lactis

Released:
DOI: 10.2210/pdb2o20/pdb
PDB entry 2o20 coloured by chain, front view.
PDB entry 2o20 coloured by chain, side view.
PDB entry 2o20 coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
Structure of the transcription regulator CcpA from Lactococcus lactis.
Loll B, Kowalczyk M, Alings C, Chieduch A, Bardowski J, Saenger W, Biesiadka J
Acta Crystallogr D Biol Crystallogr 63 431-6 (2007)
PMID: 17372346

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189959 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catabolite control protein A Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 332 amino acids
Theoretical weight: 36.64 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9CF33 (Residues: 1-332; Coverage: 100%)
Gene names: L0143, ccpA
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 117.756Å b: 74.267Å c: 160.302Å
α: 90° β: 102.36° γ: 90°
R-values:
R R work R free
0.177 0.176 0.211
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Elimination of carbon catabolite repression in Clostridium acetobutylicum--a journey toward simultaneous use of xylose and glucose.
Bruder et al. (2015)
3 more

1 mention without citation

Ultrasound-assisted extraction of emodin from Rheum officinale Baill and its antibacterial mechanism against Streptococcus suis based on CcpA.
Bai et al. (2024)