2nwp Summary

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Structural and kinetic effects of hydrophobic mutations in the active site of human carbonic anhydrase II

The structure was published by Fisher, S.Z., Tu, C.K., Bhatt, D., et al., Agbandje-McKenna, M., McKenna, R., and Silverman, D.N., in 2007 in a paper entitled "Speeding Up Proton Transfer in a Fast Enzyme: Kinetic and Crystallographic Studies on the Effect of Hydrophobic Amino Acid Substitutions in the Active Site of Human Carbonic Anhydrase II." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.8 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of carbonic anhydrase 2. This molecule has the UniProt identifier P00918 (CAH2_HUMAN)search. The sample contained 260 residues which is 100% of the natural sequence. Out of 260 residues 257 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A carbonic anhydrase 2 P00918 (1-259) (CAH2_HUMAN)search Homo sapienssearch 98% 260 99%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P00918 (1 - 259) carbonic anhydrase 2 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (P00918) Carbonic Anhydrase IIsearch PF00194: Eukaryotic-type carbonic anhydrasesearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
A (P00918) carbonate dehydratase activitysearch lyase activitysearch zinc ion bindingsearch metal ion bindingsearch protein bindingsearch plasma membranesearch extracellular spacesearch cytosolsearch cytoplasmsearch axonsearch myelin sheathsearch basolateral plasma membranesearch microvillussearch apical part of cellsearch extracellular vesicular exosomesearch membranesearch kidney developmentsearch positive regulation of osteoclast differentiationsearch positive regulation of synaptic transmission, GABAergicsearch odontogenesis of dentin-containing toothsearch positive regulation of bone resorptionsearch one-carbon metabolic processsearch positive regulation of cellular pH reductionsearch regulation of anion transportsearch regulation of chloride transportsearch response to pHsearch bicarbonate transportsearch response to estrogensearch positive regulation of dipeptide transmembrane transportsearch secretionsearch regulation of intracellular pHsearch response to steroid hormonesearch angiotensin-activated signaling pathwaysearch small molecule metabolic processsearch response to zinc ionsearch morphogenesis of an epitheliumsearch response to organic substancesearch

Chain InterPro annotation
A Alpha carbonic anhydrasesearch Carbonic anhydrase, alpha-class, conserved sitesearch Carbonic anhydrase 2search Carbonic anhydrase, alpha-classsearch