2nwo Summary

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Structural and kinetic effect of hydrophobic mutations in the active site of human carbonic anhydrase II

The structure was published by Fisher, S.Z., Tu, C.K., Bhatt, D., et al., Agbandje-McKenna, M., McKenna, R., and Silverman, D.N., in 2007 in a paper entitled "Speeding Up Proton Transfer in a Fast Enzyme: Kinetic and Crystallographic Studies on the Effect of Hydrophobic Amino Acid Substitutions in the Active Site of Human Carbonic Anhydrase II." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.7 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Carbonic anhydrase 2. This molecule has the UniProt identifier P00918 (CAH2_HUMAN)search. The sample contained 260 residues which is 100% of the natural sequence. Out of 260 residues 257 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Carbonic anhydrase 2 P00918 (1-259) (CAH2_HUMAN)search Homo sapienssearch 98% 260 99%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P00918 (1 - 259) Carbonic anhydrase 2 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (P00918) Carbonic Anhydrase IIsearch PF00194: Eukaryotic-type carbonic anhydrasesearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (P00918) one-carbon metabolic processsearch response to steroid hormonesearch angiotensin-activated signaling pathwaysearch regulation of anion transportsearch small molecule metabolic processsearch bicarbonate transportsearch positive regulation of cellular pH reductionsearch regulation of chloride transportsearch positive regulation of synaptic transmission, GABAergicsearch regulation of intracellular pHsearch response to zinc ionsearch response to organic substancesearch positive regulation of bone resorptionsearch positive regulation of dipeptide transmembrane transportsearch cellular response to fluid shear stresssearch response to pHsearch odontogenesis of dentin-containing toothsearch morphogenesis of an epitheliumsearch secretionsearch positive regulation of osteoclast differentiationsearch response to estrogensearch kidney developmentsearch zinc ion bindingsearch metal ion bindingsearch carbonate dehydratase activitysearch protein bindingsearch lyase activitysearch microvillussearch axonsearch membranesearch myelin sheathsearch cytoplasmsearch plasma membranesearch cytosolsearch extracellular vesicular exosomesearch extracellular spacesearch apical part of cellsearch basolateral plasma membranesearch

Chain InterPro annotation
A Alpha carbonic anhydrasesearch Carbonic anhydrase, alpha-class, conserved sitesearch Carbonic anhydrase 2search Carbonic anhydrase, alpha-classsearch