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EBI PDBe PDBeView

PDBe Entry: 2nvq view

RNA Polymerase II Elongation Complex in 150 mM Mg+2 with 2'dUTP
Summary
Header TRANSCRIPTION,TRANSFERASE/DNA-RNA HYBRIDsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 2.9 Å, R-factor: 23.0%, Free R-factor: 28.3%, Spacegroup: C 1 2 1
Released 12/12/2006, deposition: 13/11/2006, last revision: 24/02/2009
Authors Wang, D.search; Bushnell, D.A.search; Westover, K.D.search; Kaplan, C.D.search; Kornberg, R.D.search
Primary citation Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis
CELL(CAMBRIDGE,MASS.)search vol:127, pag:941-954 (2006) [PubMed ID 17129781 ]search
Keywords TRANSCRIPTIONsearch, MRNAsearch, MULTIPROTEIN COMPLEXsearch, MOLECULAR MACHINEsearch, DNAsearch, TRANSCRIPTIONsearch, TRANSFERASE/DNA-RNA HYBRID COMPLEXsearch
EC 2.7.7.6 ExPASy BRENDA search (A B)
Organism Saccharomyces cerevisiae(baker's yeast) 4932search(A B C E F H I J K L)
UniProt DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6) (RNA polymerase II subunit B1) (RNA polymerase II subunit 1) (DNA-directed RNA polymerase III largest subunit) (B220) P04050search (A)
DNA-directed RNA polymerase II subunit RPB2 (EC 2.7.7.6) (RNA polymerase II subunit 2) (DNA-directed RNA polymerase II 140 kDa polypeptide) (B150) P08518search (B)
DNA-directed RNA polymerase II subunit RPB3 (RNA polymerase II subunit B3) (RNA polymerase II subunit 3) (DNA-directed RNA polymerase II 45 kDa polypeptide) (B44.5) P16370search (C)
DNA-directed RNA polymerases I, II, and III subunit RPABC1 (RNA polymerases I, II, and III subunit ABC1) (DNA-directed RNA polymerases I, II, and III 27 kDa polypeptide) (ABC27) P20434search (E)
DNA-directed RNA polymerases I, II, and III subunit RPABC2 (RNA polymerases I, II, and III subunit ABC2) (DNA-directed RNA polymerases I, II, and III 23 kDa polypeptide) (ABC23) P20435search (F)
DNA-directed RNA polymerases I, II, and III subunit RPABC3 (RNA polymerases I, II, and III subunit ABC3) (DNA-directed RNA polymerases I, II, and III 14.5 kDa polypeptide) (ABC14.5) (ABC14.4) P20436search (H)
DNA-directed RNA polymerase II subunit RPB9 (RNA polymerase II subunit B9) (DNA-directed RNA polymerase II subunit 9) (DNA-directed RNA polymerase II 14.2 kDa polypeptide) (B12.6) P27999search (I)
DNA-directed RNA polymerases I, II, and III subunit RPABC5 (RNA polymerases I, II, and III subunit ABC5) (DNA-directed RNA polymerases I, II, and III 8.3 kDa polypeptide) (ABC10-beta) (ABC8) P22139search (J)
DNA-directed RNA polymerase II subunit RPB11 (RNA polymerase II subunit B11) (DNA-directed RNA polymerase II 13.6 kDa polypeptide) (B13.6) P38902search (K)
DNA-directed RNA polymerases I, II, and III subunit RPABC4 (RNA polymerases I, II, and III subunit ABC4) (ABC10-alpha) P40422search (L)
Related entries 1sfo, 1r9t, 1r9s, 2e2h, 2e2i, 2e2j, 2nvs, 2nvt, 2nvx, 2nvy, 2nvz
Polymers
Id Name Type UniProt Residues Observed
R 5'-R(*AP*UP*CP*GP*AP*GP*AP*GP*GP*A)-3' RNA
10 100%
T 28-MER DNA template strand DNA
28 100%
N 5'-D(*CP*TP*GP*CP*TP*TP*AP*TP*CP*GP*GP*TP*AP*G)-3' DNA
14 100%
A DNA-directed RNA polymerase II largest subunit Protein P04050 (RPB1_YEAST)search
 1733 81%
B DNA-directed RNA polymerase II 140 kDa polypeptide Protein P08518 (RPB2_YEAST)search
 1224 91%
C DNA-directed RNA polymerase II 45 kDa polypeptide Protein P16370 (RPB3_YEAST)search
 318 83%
E DNA-directed RNA polymerases I, II, and III 27 kDa polypeptide Protein P20434 (RPAB1_YEAST)search
 215 99%
F DNA-directed RNA polymerases I, II, and III 23 kDa polypeptide Protein P20435 (RPAB2_YEAST)search
 155 54%
H DNA-directed RNA polymerases I, II, and III 14.5 kDa polypeptide Protein P20436 (RPAB3_YEAST)search
 146 91%
I DNA-directed RNA polymerase II subunit 9 Protein P27999 (RPB9_YEAST)search
 122 97%
J DNA-directed RNA polymerases I/II/III subunit 10 Protein P22139 (RPAB5_YEAST)search
 70 92%
K DNA-directed RNA polymerase II 13.6 kDa polypeptide Protein P38902 (RPB11_YEAST)search
 120 95%
L DNA-directed RNA polymerases I, II, and III 7.7 kDa polypeptide Protein P40422 (RPAB4_YEAST)search
 70 65%
Heterogens
Id Name Ligands
A, B, C, I, J, L ZINC ION ZN search
A MAGNESIUM ION MG search
T DEOXYURIDINE-5'-TRIPHOSPHATE DUT search
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