2nuu Summary

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Regulating the Escherichia coli ammonia channel: the crystal structure of the AmtB-GlnK complex

The structure was published by Conroy, M.J., Durand, A., Lupo, D., et al., Bullough, P.A., Winkler, F.K., and Merrick, M., in 2007 in a paper entitled "The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.5 Å and deposited in 2006.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Ammonia channel and Nitrogen regulatory protein P-II 2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Ammonia channel P69681 (25-428) (AMTB_ECOLI)search Escherichia coli K-12search 95% 415 98%
B Ammonia channel P69681 (25-428) (AMTB_ECOLI)search Escherichia coli K-12search 95% 415 98%
C Ammonia channel P69681 (25-428) (AMTB_ECOLI)search Escherichia coli K-12search 95% 415 98%
D Ammonia channel P69681 (25-428) (AMTB_ECOLI)search Escherichia coli K-12search 95% 415 98%
E Ammonia channel P69681 (25-428) (AMTB_ECOLI)search Escherichia coli K-12search 95% 415 98%
F Ammonia channel P69681 (25-428) (AMTB_ECOLI)search Escherichia coli K-12search 95% 415 98%
G Nitrogen regulatory protein P-II 2 P0AC55 (1-112) (GLNK_ECOLI)search Escherichia coli K-12search 100% 112 100%
H Nitrogen regulatory protein P-II 2 P0AC55 (1-112) (GLNK_ECOLI)search Escherichia coli K-12search 100% 112 100%
I Nitrogen regulatory protein P-II 2 P0AC55 (1-112) (GLNK_ECOLI)search Escherichia coli K-12search 100% 112 100%
J Nitrogen regulatory protein P-II 2 P0AC55 (1-112) (GLNK_ECOLI)search Escherichia coli K-12search 100% 112 100%
K Nitrogen regulatory protein P-II 2 P0AC55 (1-112) (GLNK_ECOLI)search Escherichia coli K-12search 100% 112 100%
L Nitrogen regulatory protein P-II 2 P0AC55 (1-112) (GLNK_ECOLI)search Escherichia coli K-12search 100% 112 100%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P69681 (25 - 428) Ammonia channel Escherichia coli
P0AC55 (1 - 112) Nitrogen regulatory protein P-II 2 Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C, D, E, F (P69681) Ammonium transportersearch Ammonium transporter AmtB like domainssearch PF00909: Ammonium Transporter Familysearch
G, H, I, J, K, L (P0AC55) Prokaryotic signal transducing proteinsearch Alpha-Beta Plaitssearch PF00543: Nitrogen regulatory protein P-IIsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, B, C, D, E, F (P69681) ammonium transportsearch ammonium transmembrane transportsearch transportsearch carbon dioxide transportsearch ammonium transmembrane transporter activitysearch uniporter activitysearch membranesearch plasma membranesearch integral component of membranesearch integral component of plasma membranesearch
G, H, I, J, K, L (P0AC55) regulation of nitrogen utilizationsearch transcription, DNA-templatedsearch regulation of transcription, DNA-templatedsearch regulation of catalytic activitysearch positive regulation of nitrogen utilizationsearch enzyme regulator activitysearch protein bindingsearch identical protein bindingsearch nucleotide bindingsearch

Chain InterPro annotation
A, B, C, D, E, F Ammonium transportersearch Ammonium transporter, conserved sitesearch Ammonium transporter AmtB-like domainsearch Ammonium/urea transportersearch
G, H, I, J, K, L Nitrogen regulatory protein PIIsearch Nitrogen regulatory protein P-II, urydylation sitesearch Nitrogen regulatory PII-like, alpha/betasearch Nitrogen regulatory protein PII/ATP phosphoribosyltransferase, C-terminalsearch Nitrogen regulatory protein PII, conserved sitesearch