› 2nuf

X-ray diffraction
2.5Å resolution

Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA at 2.5-Angstrom Resolution

Released:
DOI: 10.2210/pdb2nuf/pdb
PDB entry 2nuf coloured by chain, front view.
PDB entry 2nuf coloured by chain, side view.
PDB entry 2nuf coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
A stepwise model for double-stranded RNA processing by ribonuclease III.
Gan J, Shaw G, Tropea JE, Waugh DS, Court DL, Ji X
Mol Microbiol 67 143-54 (2008)
PMID: 18047582

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130437 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 26.15 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67082 (Residues: 1-221; Coverage: 100%)
Gene names: aq_946, rnc
Sequence domains:
Structure domains:
28-MER Chains: C, D
Molecule details ›
Chains: C, D
Length: 28 nucleotides
Theoretical weight: 9.01 KDa
Source organism: Aquifex aeolicus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 64.1Å b: 51.1Å c: 113.4Å
α: 90° β: 104.9° γ: 90°
R-values:
R R work R free
0.226 0.222 0.284
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
13 more

9 mentions without citation

RNA recognition by double-stranded RNA binding domains: a matter of shape and sequence.
Masliah et al. (2013)
8 more