2nt1

X-ray diffraction
2.3Å resolution

Structure of acid-beta-glucosidase at neutral pH

Released:

Function and Biology Details

Reactions catalysed:
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
PDBe Complex ID:
PDB-CPX-137377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysosomal acid glucosylceramidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 497 amino acids
Theoretical weight: 55.64 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04062 (Residues: 40-536; Coverage: 100%)
Gene names: GBA, GBA1, GC, GLUC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 110.185Å b: 91.792Å c: 153.036Å
α: 90° β: 110.91° γ: 90°
R-values:
R R work R free
0.178 0.175 0.24
Expression system: Cricetulus griseus