› 2nrn

X-ray diffraction
1.4Å resolution

Self-assembly of coiled-coil tetramers in the 1.40 A structure of a leucine-zipper mutant

Released:
DOI: 10.2210/pdb2nrn/pdb
PDB entry 2nrn coloured by chain, front view.
PDB entry 2nrn coloured by chain, side view.
PDB entry 2nrn coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Self-assembly of coiled-coil tetramers in the 1.40 A structure of a leucine-zipper mutant.
Deng Y, Zheng Q, Liu J, Cheng CS, Kallenbach NR, Lu M
Protein Sci 16 323-8 (2007)
PMID: 17189475

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136532 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 34 amino acids
Theoretical weight: 3.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P03069 (Residues: 248-281; Coverage: 12%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C
Sequence domains: Basic region leucine zipper

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 20.117Å b: 87.875Å c: 35.509Å
α: 90° β: 103.83° γ: 90°
R-values:
R R work R free
0.185 0.183 0.219
Expression system: Escherichia coli BL21

Quick links

Citations

10 citation in other articles

Essential role of coiled coils for aggregation and activity of Q/N-rich prions and PolyQ proteins.
Fiumara et al. (2010)
9 more

2 mentions without citation

Self-assembly of coiled-coil tetramers in the 1.40 A structure of a leucine-zipper mutant.
Deng et al. (2007)
1 more