› 2m07

Solution NMR

NMR structure of OmpX in DPC micelles

Released:
DOI: 10.2210/pdb2m07/pdb
PDB entry 2m07 coloured by chain, ensemble of 20 models, front view.
PDB entry 2m07 coloured by chain, ensemble of 20 models, side view.
PDB entry 2m07 coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli K-12
Primary publication:
Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins.
Hagn F, Etzkorn M, Raschle T, Wagner G
J Am Chem Soc 135 1919-25 (2013)
PMID: 23294159

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein X Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.4 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A917 (Residues: 24-171; Coverage: 100%)
Gene names: JW0799, b0814, ompX, ybiG
Sequence domains: Outer membrane protein beta-barrel domain
Structure domains: Porin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 36%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21

Quick links

Citations

43 review citations

Nanodiscs in Membrane Biochemistry and Biophysics.
Denisov et al. (2017)
42 more

5 mentions without citation

Perturbations of Native Membrane Protein Structure in Alkyl Phosphocholine Detergents: A Critical Assessment of NMR and Biophysical Studies.
Chipot et al. (2018)
4 more