› 2lt7

Solution NMR

Solution NMR structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA

Released:
DOI: 10.2210/pdb2lt7/pdb
PDB entry 2lt7 coloured by chain, ensemble of 20 models, front view.
PDB entry 2lt7 coloured by chain, ensemble of 20 models, side view.
PDB entry 2lt7 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for recognition of methylated and specific DNA sequences by the zinc finger protein Kaiso.
Buck-Koehntop BA, Stanfield RL, Ekiert DC, Martinez-Yamout MA, Dyson HJ, Wilson IA, Wright PE
Proc Natl Acad Sci U S A 109 15229-34 (2012)
PMID: 22949637

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115564 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional regulator Kaiso Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 16.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86T24 (Residues: 472-604; Coverage: 20%)
Gene names: KAISO, ZBTB33, ZNF348
Structure domains: Classic Zinc Finger
DNA (5'-D(*GP*TP*GP*CP*TP*TP*CP*CP*TP*GP*CP*CP*AP*AP*TP*AP*AP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.78 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*GP*TP*TP*AP*TP*TP*GP*GP*CP*AP*GP*GP*AP*AP*GP*CP*AP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 19 nucleotides
Theoretical weight: 5.87 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 73%
Refinement method: molecular dynamics
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

23 review citations

Interpreting the language of histone and DNA modifications.
Rothbart et al. (2014)
22 more

3 mentions without citation

Molecular basis for recognition of methylated and specific DNA sequences by the zinc finger protein Kaiso.
Buck-Koehntop et al. (2012)
2 more