› 2lmd

Solution NMR

Minimal Constraints Solution NMR Structure of Prospero Homeobox protein 1 from Homo sapiens, Northeast Structural Genomics Consortium Target HR4660B

Released:
DOI: 10.2210/pdb2lmd/pdb
PDB entry 2lmd coloured by chain, ensemble of 20 models, front view.
PDB entry 2lmd coloured by chain, ensemble of 20 models, side view.
PDB entry 2lmd coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Determination of solution structures of proteins up to 40 kDa using CS-Rosetta with sparse NMR data from deuterated samples.
Lange OF, Rossi P, Sgourakis NG, Song Y, Lee HW, Aramini JM, Ertekin A, Xiao R, Acton TB, Montelione GT, Baker D
Proc Natl Acad Sci U S A 109 10873-8 (2012)
PMID: 22733734

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prospero homeobox protein 1 Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92786 (Residues: 575-737; Coverage: 22%)
Gene name: PROX1
Sequence domains: Homeo-prospero domain
Structure domains: Homeo-prospero domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 55%
Refinement method: molecular dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.
Currin et al. (2015)
14 more

4 mentions without citation

A community resource of experimental data for NMR / X-ray crystal structure pairs.
Everett et al. (2016)
3 more