› 2kxh

Solution NMR

Solution structure of the first two RRM domains of FIR in the complex with FBP Nbox peptide

Released:
DOI: 10.2210/pdb2kxh/pdb
PDB entry 2kxh coloured by chain, ensemble of 20 models, front view.
PDB entry 2kxh coloured by chain, ensemble of 20 models, side view.
PDB entry 2kxh coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of FIR-mediated c-myc transcriptional control.
Cukier CD, Hollingworth D, Martin SR, Kelly G, Díaz-Moreno I, Ramos A
Nat Struct Mol Biol 17 1058-64 (2010)
PMID: 20711187

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188355 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Poly(U)-binding-splicing factor PUF60 Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 21.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHX1 (Residues: 119-314; Coverage: 35%)
Gene names: FIR, PUF60, ROBPI, SIAHBP1
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Far upstream element-binding protein 1 Chain: B
Molecule details ›
Chain: B
Length: 31 amino acids
Theoretical weight: 3.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96AE4 (Residues: 27-52; Coverage: 4%)
Gene name: FUBP1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Single-stranded DNA-binding proteins: multiple domains for multiple functions.
Dickey et al. (2013)
6 more

3 mentions without citation

Molecular basis of FIR-mediated c-myc transcriptional control.
Cukier et al. (2010)
2 more