› 2klv

Solution NMR

Membrane-bound structure of the Pf1 major coat protein in DHPC micelle

Released:
DOI: 10.2210/pdb2klv/pdb
PDB entry 2klv coloured by chain, ensemble of 15 models, front view.
PDB entry 2klv coloured by chain, ensemble of 15 models, side view.
PDB entry 2klv coloured by chain, ensemble of 15 models, top view.
Source organism: Pseudomonas virus Pf1
Primary publication:
Phage-induced alignment of membrane proteins enables the measurement and structural analysis of residual dipolar couplings with dipolar waves and lambda-maps.
Park SH, Son WS, Mukhopadhyay R, Valafar H, Opella SJ
J Am Chem Soc 131 14140-1 (2009)
PMID: 19761238

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137182 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid protein G8P Chain: A
Molecule details ›
Chain: A
Length: 46 amino acids
Theoretical weight: 4.61 KDa
Source organism: Pseudomonas virus Pf1
Expression system: Pseudomonas aeruginosa
UniProt:
  • Canonical: P03621 (Residues: 37-82; Coverage: 85%)
Gene name: VIII
Sequence domains: Inovirus Coat protein B
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Pseudomonas aeruginosa

Quick links

Citations

3 review citations

Comparative NMR studies demonstrate profound differences between two viroporins: p7 of HCV and Vpu of HIV-1.
Cook et al. (2011)
2 more

3 mentions without citation

Hepatitis C virus RNA replication requires a conserved structural motif within the transmembrane domain of the NS5B RNA-dependent RNA polymerase.
Brass et al. (2010)
2 more