› 2k7f

Solution NMR

HADDOCK calculated model of the complex between the BRCT region of RFC p140 and dsDNA

Released:
DOI: 10.2210/pdb2k7f/pdb
PDB entry 2k7f coloured by chain, ensemble of 4 models, front view.
PDB entry 2k7f coloured by chain, ensemble of 4 models, side view.
PDB entry 2k7f coloured by chain, ensemble of 4 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the DNA-bound BRCA1 C-terminal region from human replication factor C p140 and model of the protein-DNA complex.
Kobayashi M, Ab E, Bonvin AMJJ, Siegal G
J Biol Chem 285 10087-10097 (2010)
PMID: 20081198

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115454 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Replication factor C subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35251 (Residues: 375-480; Coverage: 9%)
Gene names: RFC1, RFC140
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain
5'-D(P*DCP*DGP*DAP*DCP*DCP*DTP*DCP*DGP*DAP*DGP*DAP*DTP*DCP*DA)-3' Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.25 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(P*DCP*DTP*DCP*DGP*DAP*DGP*DGP*DTP*DCP*DG)-3' Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3.06 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: HADDOCK
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

BRCT domains: easy as one, two, three.
Leung et al. (2011)
3 more

4 mentions without citation

Structure of the DNA-bound BRCA1 C-terminal region from human replication factor C p140 and model of the protein-DNA complex.
Kobayashi et al. (2010)
3 more