› 2jzb

Solution NMR

Solution structure of the complex between E.coli NusA-AR2 and RNAP-aCTD

Released:
DOI: 10.2210/pdb2jzb/pdb
PDB entry 2jzb coloured by chain, ensemble of 20 models, front view.
PDB entry 2jzb coloured by chain, ensemble of 20 models, side view.
PDB entry 2jzb coloured by chain, ensemble of 20 models, top view.
Source organisms:
Entry authors: Prasch S, Schweimer K, Roesch P

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142714 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-directed RNA polymerase subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 99 amino acids
Theoretical weight: 11.12 KDa
Source organism: Yersinia pseudotuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q664U6 (Residues: 233-329; Coverage: 30%)
Gene names: YPTB3673, rpoA
Sequence domains: Bacterial RNA polymerase, alpha chain C terminal domain
Structure domains: 5' to 3' exonuclease, C-terminal subdomain
Transcription termination/antitermination protein NusA Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 7.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFF6 (Residues: 424-495; Coverage: 15%)
Gene names: JW3138, b3169, nusA
Sequence domains: Helix-hairpin-helix domain
Structure domains: 5' to 3' exonuclease, C-terminal subdomain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 81%
Refinement method: simulated annealing
Expression system: Escherichia coli

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