› 2jyb

Solution NMR

binary hvDHFR1:folate complex

Released:
DOI: 10.2210/pdb2jyb/pdb
PDB entry 2jyb coloured by chain, ensemble of 20 models, front view.
PDB entry 2jyb coloured by chain, ensemble of 20 models, side view.
PDB entry 2jyb coloured by chain, ensemble of 20 models, top view.
Source organism: Haloferax volcanii
Primary publication:
NMR-derived folate-bound structure of dihydrofolate reductase 1 from the halophile Haloferax volcanii.
Boroujerdi AF, Young JK
Biopolymers 91 140-4 (2009)
PMID: 18825778

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147214 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase HdrA Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 17.98 KDa
Source organism: Haloferax volcanii
Expression system: Escherichia coli
UniProt:
  • Canonical: P15093 (Residues: 1-162; Coverage: 100%)
Gene names: C498_12348, HVO_1279, folA, hdrA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Gates of enzymes.
Gora et al. (2013)
1 more