› 2jbp

X-ray diffraction
3.31Å resolution

Protein kinase MK2 in complex with an inhibitor (crystal form-2, co- crystallization)

Released:
DOI: 10.2210/pdb2jbp/pdb
PDB entry 2jbp coloured by chain, front view.
PDB entry 2jbp coloured by chain, side view.
PDB entry 2jbp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for a high affinity inhibitor bound to protein kinase MK2.
Hillig RC, Eberspaecher U, Monteclaro F, Huber M, Nguyen D, Mengel A, Muller-Tiemann B, Egner U
J Mol Biol 369 735-45 (2007)
PMID: 17449059

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155865 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MAP kinase-activated protein kinase 2 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 326 amino acids
Theoretical weight: 37.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P49137 (Residues: 41-364; Coverage: 81%)
Gene name: MAPKAPK2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand P4O 11 x P4O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 139.983Å b: 215.564Å c: 179.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.279
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Targeting innate immunity protein kinase signalling in inflammation.
Gaestel et al. (2009)
4 more

6 mentions without citation

Targeting Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2, MK2): Medicinal Chemistry Efforts To Lead Small Molecule Inhibitors to Clinical Trials.
Fiore et al. (2016)
5 more