2jb0

X-ray diffraction
1.91Å resolution

CRYSTAL STRUCTURE OF THE MUTANT H573A OF THE NUCLEASE DOMAIN OF COLE7 IN COMPLEX WITH IM7

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-169937 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E7 immunity protein Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 9.91 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03708 (Residues: 1-87; Coverage: 100%)
Gene names: ceiE7, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E7 Chain: B
Molecule details ›
Chain: B
Length: 131 amino acids
Theoretical weight: 15 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47112 (Residues: 446-576; Coverage: 23%)
Gene names: cea, colE7
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: I222
Unit cell:
a: 64.118Å b: 74.367Å c: 119.612Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.25
Expression system: Escherichia coli