› 2j7q

X-ray diffraction
1.8Å resolution

Crystal structure of the ubiquitin-specific protease encoded by murine cytomegalovirus tegument protein M48 in complex with a ubquitin-based suicide substrate

Released:
DOI: 10.2210/pdb2j7q/pdb
PDB entry 2j7q coloured by chain, front view.
PDB entry 2j7q coloured by chain, side view.
PDB entry 2j7q coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes.
Schlieker C, Weihofen WA, Frijns E, Kattenhorn LM, Gaudet R, Ploegh HL
Mol Cell 25 677-87 (2007)
PMID: 17349955

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143370 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
MCMV TEGUMENT PROTEIN M48 ENCODED UBIQUITIN- SPECIFIC PROTEASE, M48USP Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.68 KDa
Source organism: Murid betaherpesvirus 1
Expression system: Escherichia coli BL21(DE3)
Structure domains: Cathepsin B; Chain A
Ubiquitin Chains: B, D
Molecule details ›
Chains: B, D
Length: 75 amino acids
Theoretical weight: 8.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-683; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
MCMV TEGUMENT PROTEIN M48 ENCODED UBIQUITIN- SPECIFIC PROTEASE, M48USP Chain: C
Molecule details ›
Chain: C
Length: 232 amino acids
Theoretical weight: 25.69 KDa
Source organism: Murid betaherpesvirus 1
Expression system: Escherichia coli BL21(DE3)
Structure domains: Cathepsin B; Chain A

Ligands and Environments

4 bound ligands:
Ligand MG 3 x MG
Ligand GVE 2 x GVE
Ligand PG4 1 x PG4
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 40.907Å b: 57.298Å c: 67.279Å
α: 73.37° β: 85.37° γ: 88.54°
R-values:
R R work R free
0.157 0.156 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Ubiquitination, ubiquitin-like modifiers, and deubiquitination in viral infection.
Isaacson et al. (2009)
16 more

12 mentions without citation

Structure and Function of Viral Deubiquitinating Enzymes.
Bailey-Elkin et al. (2017)
11 more