› 2j59

X-ray diffraction
2.1Å resolution

Crystal structure of the ARF1:ARHGAP21-ArfBD complex

Released:
DOI: 10.2210/pdb2j59/pdb
PDB entry 2j59 coloured by chain, front view.
PDB entry 2j59 coloured by chain, side view.
PDB entry 2j59 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi membranes.
Ménétrey J, Perderiset M, Cicolari J, Dubois T, Elkhatib N, El Khadali F, Franco M, Chavrier P, Houdusse A
EMBO J 26 1953-62 (2007)
PMID: 17347647

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160800 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ADP-ribosylation factor 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 166 amino acids
Theoretical weight: 18.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84078 (Residues: 18-181; Coverage: 91%)
Gene name: Arf1
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Rho GTPase-activating protein 21 Chains: M, N, O, P, Q, R
Molecule details ›
Chains: M, N, O, P, Q, R
Length: 168 amino acids
Theoretical weight: 19.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5T5U3 (Residues: 930-1097; Coverage: 9%)
Gene names: ARHGAP10, ARHGAP21, KIAA1424
Sequence domains: PH domain
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand GTP 6 x GTP
Ligand MG 6 x MG
Ligand DIO 3 x DIO
Ligand EDO 3 x EDO
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21
Unit cell:
a: 74.636Å b: 132.136Å c: 146.283Å
α: 90° β: 90.01° γ: 90°
R-values:
R R work R free
0.209 0.205 0.243
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
11 more

11 mentions without citation

Dynamic structure of membrane-anchored Arf*GTP.
Liu et al. (2010)
10 more