› 2j37

Electron Microscopy
8Å resolution

MODEL OF MAMMALIAN SRP BOUND TO 80S RNCS

Released:
DOI: 10.2210/pdb2j37/pdb
PDB entry 2j37 coloured by chain, front view.
PDB entry 2j37 coloured by chain, side view.
PDB entry 2j37 coloured by chain, top view.
Source organisms:
Primary publication:
Following the signal sequence from ribosomal tunnel exit to signal recognition particle.
Halic M, Blau M, Becker T, Mielke T, Pool MR, Wild K, Sinning I, Beckmann R
Nature 444 507-11 (2006)
PMID: 17086193
Related structures: EMD-1264

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-131345 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (8 distinct):
Ribosomal protein L25 Chain: 4
Molecule details ›
Chain: 4
Length: 152 amino acids
Theoretical weight: 16.92 KDa
Source organism: Triticum sp.
UniProt:
  • Canonical: O81229 (Residues: 1-109; Coverage: 100%)
Sequence domains: Ribosomal protein L23
Large ribosomal subunit protein uL29 Chain: 5
Molecule details ›
Chain: 5
Length: 124 amino acids
Theoretical weight: 14.4 KDa
Source organism: Triticum sp.
UniProt:
  • Canonical: Q8L805 (Residues: 1-124; Coverage: 100%)
Gene name: RPL35
Sequence domains: Ribosomal L29 protein
Putative 60S ribosomal protein L31 Chain: 6
Molecule details ›
Chain: 6
Length: 123 amino acids
Theoretical weight: 14.15 KDa
Source organism: Triticum sp.
UniProt:
  • Canonical: Q5XLD9 (Residues: 1-124; Coverage: 99%)
Sequence domains: Ribosomal protein L31e
Signal recognition particle 19 kDa protein Chain: B
Molecule details ›
Chain: B
Length: 108 amino acids
Theoretical weight: 12.56 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P09132 (Residues: 14-120; Coverage: 74%)
Gene name: SRP19
Sequence domains: SRP19 protein
G-protein coupled receptors family 1 profile domain-containing protein Chain: S
Molecule details ›
Chain: S
Length: 17 amino acids
Theoretical weight: 1.99 KDa
Source organism: Canis sp.
UniProt:
  • Canonical: Q1WHN3 (Residues: 43-59; Coverage: 16%)
Signal recognition particle subunit SRP54 Chain: W
Molecule details ›
Chain: W
Length: 504 amino acids
Theoretical weight: 55.78 KDa
Source organism: Canis sp.
UniProt:
  • Canonical: P61010 (Residues: 1-504; Coverage: 100%)
Gene name: SRP54
Sequence domains:
SRP RNA Chain: A
Molecule details ›
Chain: A
Length: 128 nucleotides
Theoretical weight: 41.5 KDa
Sequence domains: Metazoan signal recognition particle RNA
RIBOSOMAL RNA Chain: Z
Molecule details ›
Chain: Z
Length: 280 nucleotides
Theoretical weight: 90.7 KDa
Sequence domains: Archaeal large subunit ribosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8Å
Relevant EMDB volumes: EMD-1264

Quick links

Citations

42 review citations

Protein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes.
Rapoport TA. (2007)
41 more

7 mentions without citation

Recognition of a signal peptide by the signal recognition particle.
Janda et al. (2010)
6 more